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Progesterone Receptor (PGR) content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. PGR clone 16 is directed against the human progesterone receptor molecule. A prokaryotic recombinant protein, corresponding to the N-terminal region of the A form of human progesterone receptor, was used as the immunogen. Antibody characterization studies demonstrated that PGR Clone 16 reacts with both A and B forms of human progesterone receptor in Western blotting procedure.